Efecto de la transición timina/citosina del gen BGH sobre parámetros reproductivos en ganado Holstein en Colombia
Resumen
La rentabilidad de los hatos lecheros depende en gran medida del mantenimiento de una alta eficiencia reproductiva. Una reproducción adecuada permite mayor producción de leche durante la vida del animal. El objetivo del trabajo fue determinar la asociación del polimorfismo del intrón 3 de la hormona de crecimiento bovino (BGH), con el número de servicios, días abiertos e intervalo entre partos en vacas de raza Holstein del departamento de Antioquia. El estudio se realizó con 415 vacas, ubicadas en 8 municipios del departamento de Antioquia. La genotipificación se llevó a cabo usando la técnica de PCR-RFLP con DNA extraído de sangre periférica mediante la técnica de salting out. La información fenotípica utilizada, fue recopilada durante 4 años, a partir de un programa de control de producción lechera vigente en los hatos. Para la asociación se realizaron análisis estadísticos con métodos paramétricos. Las frecuencias alélicas para los alelos (+) y (–) fueron 0,91 y 0,09. Las frecuencias genotípicas fueron (0,77), (0,2) y (0,03) para (+/+), (+/-) y (-/-) respectivamente. Se presentaron diferencias significativas entre las medias de los días abiertos, intervalo entre parto y número de servicios; para todas estas características los animales portadores del genotipo (+/+) tuvieron los parámetros menos favorables. Los resultados indican asociación del polimorfismo del intrón 3 de la hormona de crecimiento bovino con el número de servicios al primer parto, intervalo entre parto y los días abiertos facilitando la selección de estos genotipos para su utilización en programas de mejoramiento genético y reproductivo.
Descargas
Citas
• Balogh, O., K. Kovacs , M. Kulcsar , A. Gaspardy , A. Zsolnai , L. Katai , A. Pécsi, L. Fésüs , W. R. Butler and G. Huszenicza. 2009. AluI polymorphism of the bovine growth hormone (GH) gene, resumption of ovarian cyclicity, milk production and loss of body condition at the onset of lactation in dairy cows. Theriogenology.,71 (4): 553 -559. Available on line: https://bit.ly/2TyNr1J. [Ene. 12, 2015].
• Dybus, A. 2002. Associations of growth (GH) and Prolactin (PRL) genes polymorphisms with milk production traits in Polish Black and White cattle. Anim Sci P., 20 (4):203 -212. Available on line: https://bit.ly/38BBoFh. [Nov. 02, 2014].
• Gorbani, A., T. Vaez, M. Bonyadi and C. Amirinia. 2009. A MspI PCR-RFLP within bovin growth hormone gene and its association with sperm quality traits in Iranian Holstein bulls. Afr J Biotechnol., 8 (19); 4811 - 4816. Available on line: https://bit.ly/2TBGi0y . [Ene. 12, 2015].
• Gretel, R., C. Mariana, J. M. Ramos and I. Pereira. 2011. Metabolic and endocrine profiles and reproductive parameters in dairy cows under grazing conditions: effect of polymorphisms in somatotropic axis genes. Acta Veterinaria Scandinavica., 53:35. Available on line: https://bit.ly/2IvRKEu. [Dic. 18,2014].
• Hartl, D. 2000. A primer of populations Genetics. Sinauer Associates, Inc. Publishers. Sunderland, Mássachussets. U.S.A. 3ra Ed., pp. 26 - 31.
• Hayhurst C., A. Flint, P. Lovendahl and J. Woolliams. 2009. Genetic variation of metabolite and hormone concentration in UK Holstein-Friesian calves and the genetic relationship with economically important traits. J Dairy Sci., 92 (8):4001-4007. Available on line: https://bit.ly/2TRozBi. [Nov.14, 2014].
• Jiang, H. and M. C. Lucy. 2001. Variants of the 5’-untranslated region of the bovine growth hormone receptor mRNA: isolation, expression and effects on translational efficiency. Gene., 265 (1-2): 45 -53. Available on line: https://bit.ly/2VWnbQl.[Ene. 12, 2015].
• Katalin, K., V. József, Z. Attila, G. István and F. László. 2006. Associations between the AluI polymorphism of growth hormone gene and production and reproduction traits in a Hungarian Holstein-Friesian bull dam population. Arch Tierz., 49 (3): 236 -249. Available on line: https://bit.ly/3cIJOxJ. [Ene. 12,2015].
• Komisarek, J., M. Arkadiusz and W. Anna. 2011. The effects of polymorphisms in DGAT1, GH and GHR genes on reproduction and production traits in Jersey cows. Animal Science Papers and Reports, 29 (1): 29-36. Available on line: https://bit.ly/2v5pA03. [Dic.10, 2014].
• Le, D., C. Bondy , S. Yakar , J. Liu and A. Butler . 2001. The Somatomedin Hypothesis 2001. Endocr Rev., 22 (1): 53 -74. Available on line: https://bit.ly/39BpInh. [Ene. 20, 2015].
• Lee, B., G. Lin, B. Crooker, M. Murtaugh, L. Hansen and H. J. Chester. 1993. Association of somatotropin gene polymorphism at the 5th exon with selection for milk yield in holstein cows. Domest Anim Endocrin., 13 (4): 376 – 381. Available on line: https://bit.ly/3aBkHLe. [Dic.15, 2014].
• Lechniak, D., T. Adamowicz, D. Stanislawski and D. Kaczmarek. 2002. In vitro maturation and fertilization of bovine oocytes in relation to GH gene polymorphism (Leu/Val). Reprod Nutr Dev., 42 (3): 275 - 280. Available on line: https://bit.ly/2Q0NnWc. [Nov. 20, 2014].
• Lingappa, V., A. Devillers and G. Blobel. 1977. Nascent prehormones are intermediates in the biosynthesis of authentic bovine pituitary growth hormone and prolactin. P Natl A Sci., 74 (6): 2432 - 2436. Available on line: https://bit.ly/2TAJPfH.[Dic. 15, 2014].
• Lucy, M. 2008. Functional differences in the growth hormone and insulin-like growth factor axis in cattle and pigs: implications for post-partum nutrition and reproduction. Reprod Domest Anim., 43 (2): 31 - 39. Available on line: https://bit.ly/3cHl7BL. [Ene. 20, 2015].
• Miller, S., D. Dykes and H. Polesky . 1988. A simple salting-out procedure for extracting DNA from human nucleated cells. Nucleic Acid Res., 16: 1215. Available on line: https://bit.ly/2wKo6Zv. [Ene. 20, 2015].
• Mullen, M., D. Berry , D. Howard , M. Diskin, C. Lynch , E. Berkowicz , D. A. Magee, D. E. MacHugh and S. M. Waters. 2010. Associations between novel single nucleotide polymorphisms in the Bos Taurus growth hormone gene and performance traits in Holstein-Frisian dairy cattle. J Dairy Sci., 93 (12): 5959 - 5969. Available online: https://bit.ly/2PZz8kp. [Ene. 20, 2015].
• Pereira, A., M. Maurício, N. Henrique and C. Luciana. 2005. Association of GH and IGF1 polymorphisms with growth traits in a synthetic beef cattle breed. Genet Mol Biol., 28 (2): 230 - 236. Available on line: https://bit.ly/2Q22w9X . [Ene.12, 2015].
• Renaville, R., M. Hammadi and D. Portetelle . 2002. Role of the somatotropic axis in the mammalian metabolism. Domest Anim Endocrinol., 23 (1-2): 351- 360. Available online: https://bit.ly/2PYlTRh. [Ene. 12, 2015].
• Reiss, C., D. Navas, M. Pereira and A. Cravador. 2001. Growth hormone Alu polymorphism analysis in eight portuguese bovine breeds. Arch zootec., 50: 41-48. Available on line: https://bit.ly/2TRpWjq. [Ene. 20, 2015].
• Reist, M., D Erdin , E. Von , K. Tschümperlin , H. Leuenberger , H. Hammon , C. Morel, C. Philipona , Y. Zbinden, N. Künzi and J. W. Blum . 2003. Postpartum reproductive function: association with energy, metabolic and endocrine status in high yielding dairy cows. Theriogenology., 59 (8): 1707- 1723. Available on line: https://bit.ly/332PQVv. [Ene. 20, 2015].
• SAS 9.2 SQL Procedure User’s Guide. 2009.SAS Institute., Inc., Cary, N.C, USA.
• Scaramuzzi, R., J. Murray , J. Downing and B. Campbell . 1999. The effects of exogenous growth hormone on follicular steroid secretion and ovulation rate in sheep. Domest Anim Endocrinol.; 17 (2-3):269 – 277. Available on line: https://bit.ly/38AazkJ. [Enero,2015].
• Velásquez, M., L. Spicer and D. Wathes. 2008. The role of endocrine insulin-like growth factor-I (IGF-I) in female bovine reproduction. Domest Anim Endocrinol., 35 (4): 325 - 342. Available on line: https://bit.ly/333Zhnz. [Dic. 04, 2014].
• Wathes, D., M. Fenwick, Z. Cheng, N. Bourne, S. Llewellyn, D. Morris, D. Kenny, J. Murphy and R. Fitzpatrick. 2007. Influence of negative energy balance on cyclicity and fertility in the high producing dairy cow. Theriogenology., 68 (1): S232 - 41. Available on line: https://bit.ly/2xokvAW .[Dic. 04, 2014].
• Zhang, H., D. Brown and S. Denise. 1993. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism analysis of the bovine somatotropin gene. J Anim Sci., 71: 2276. Available on line: https://bit.ly/2TPuuH5. [Dic. 04, 2014].
• Zwierzchowski, L., J. Krzyzeweski, N. Strzalkowska,E. Siadkowska and Z. Ryniewicz. 2002. Effects of polymorphism of growth hormone (GH), Pit-1, and Leptin (LEP) genes, cow´s age, lactation stage and somatic cell count on milk yield and composition of polish black-and-white cows. Anim Sci P. 20 (2): 213 -227. Available on line: https://bit.ly/3aDo0BM. [Ene.12, 2015].
Derechos de autor 2015 Juliana Arango, José J. Echeverri, Albeiro López
Esta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0.
